| Position | Driver | Team | Laps | Best Time |
| 1 | S Vettel | Red Bull | 32 | 1:16.877 |
| 2 | F Alonso | Ferrari | 35 | 1:16.963 |
| 3 | Nico Rosberg | Mercedes | 34 | 1:17.151 |
| 4 | M Webber | Red Bull | 33 | 1:17.273 |
| 5 | F Massa | Ferrari | 33 | 1:17.401 |
| 6 | A Sutil | Force India | 28 | 1:17.415 |
| 7 | L Hamilton | McLaren | 29 | 1:17.522 |
| 8 | Robert Kubica | Renault | 36 | 1:17.529 |
| 9 | M Schumacher | Mercedes | 34 | 1:17.688 |
| 10 | T Liuzzi | Force India | 35 | 1:17.903 |
| 11 | J Button | McLaren | 33 | 1:17.961 |
| 12 | R Barrichello | Williams | 27 | 1:18.385 |
| 13 | N Hulkenberg | Williams | 41 | 1:18.447 |
| 14 | V Petrov | Renault | 40 | 1:18.582 |
| 15 | De La Rosa | BMW | 34 | 1:18.658 |
| 16 | K Kobhayashi | BMW | 38 | 1:19.142 |
| 17 | S Bueimi | Toro Rosso | 32 | 1:19.168 |
| 18 | J Alguesuari | Toro Rosso | 41 | 1:19.274 |
| 19 | H Kovalainen | Lotus | 35 | 1:19.969 |
| 20 | K Chandhok | HRT | 29 | 1:20.879 |
| 21 | B Senna | HRT | 31 | 1:21.097 |
| 22 | J Trulli | Lotus | 11 | 1:21.346 |
| 23 | Timo Glock | Virgin | 25 | 1:21.488 |
| 24 | Di Grazzi | Virgin | 30 | 1:21.577 |
2010 Formula 1 Canadian Grand Prix First Practice Results
